Successful Experiment Shows Homeopathic Arnica Stimulates Wound Healing Genes

Given the central role of macrophages in tissue repair and regeneration, we formulated the hypothesis that one of the cellular targets of Arnica m. an action is the macrophage and accordingly decided to evaluate this plant’s effects in vitro on the THP-1 human cell line, a widely used model for immune modulation. This cell line is widely used in laboratories for the study of macrophage biochemistry and molecular biology.

The advantage of a cell line resides essentially in the easier reproducibility of experiments in the same conditions, avoiding the variations due to individual sensitivity of different donors. Since we used very low doses of drugs—even with the highest Arnica m. 2c dilution, in the assay medium the sesquiterpene lactones are in the 10−8 Mol/L dose—we expected small effect sizes and so preferred to use a highly reproducible model. THP-1 cells resemble primary monocytes, but when treated with low doses of phorbol esters (PMA) they differentiate to cells with the morphological and functional features of tissue macrophages. On the basis of environmental cues and molecular mediators, macrophages differentiate to either a proinflammatory type (M1) or to an anti-inflammatory or pro-reparatory type (M2).

Accordingly, we used THP-1 macrophages polarized by interleukin-4 (IL-4) treatment to a phenotype that takes on characteristic properties functional to immune regulation, wound healing, and tissue remodeling. The results of this work indicate that Arnica m. acts on macrophages by modulating a number of genes and by increasing cell motility. RNA-Seq analysis allowed the identification of several genes which are particularly sensitive to ultra-low doses and high dilutions of this plant extract.

Molecular analysis of gene expression suggests that a primary action of this medicinal plant is the stimulation of tissue matrix synthesis. These findings provide new insights into wound-associated molecular events and specifically point to macrophage fibronectin production as a potential therapeutic target of Arnica m. for the treatment of wound repair.

Successful Experiment Shows Homeopathic Arnica Stimulates Wound Healing Genes

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Depression Fastest Growing Condition in UK and Homeopathy Can Help

According to a recent report in GP online(1), depression is now the fastest growing condition in the UK.

470,000 new cases were reported in 2015/16, which exceeded obesity, hypertension, and diabetes. This leaves the total number of patients over the age of 18 with recorded depression standing at 3.8m people or 8.3% of the population.

The report is based on analysis prevalence data from Quality and Outcomes Framework(QOF), published in NHS digital.2 In the meanwhile, waiting times for patients to access talking therapies such as Cognigive Behavioural Therapy is, according to the General Practitioners Committee deputy chairman Dr. Richard Vautrey unacceptably long.

Homeopathy and depression

Given that women are up to three times more likely to develop major depressive order, findings from a recent trial where homeopathy was given to peri-menopausal and menopausal women with depressive symptoms is very interesting.

This randomized, placebo controlled trial took place in Mexico and included 133 women. 54.5% of women responded positively to homeopathic treatment, which was similar to the response to fluoxetine but significantly higher than from placebo. Only homeopathic treatment led to a reduction of menopausal symptoms, which is in line with its whole person effects.

1. http://www.gponline.com/depression-fastest-growing-condition-gps-record-470000-new-cases-2015-16/mental-health/depression/article/1414006

2. http://content.digital.nhs.uk/QOF

3. Aguilar-Faisal L, Asbun-Bojalil J (2015) IndividualizedHomeopathic Treatment and Fluoxetine for Moderate to Severe Depression in Peri- and Postmenopausal Women (HOMDEP-MENOP Study): A Randomized, Double-Dummy, Double-Blind, Placebo-Controlled Trial. PLoS ONE 10(3): e0118440. doi:10.1371/journal.pone.0118440

 

Depression Fastest Growing Condition in UK and Homeopathy Can Help

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